Anti-Myelin Basic Protein Mouse mAb (SMI-99)

Application

ELISA (1:1000)Immunoblotting (1:1000)Immunocytochemistry (1:1000)Frozen Sections (1:1000)Paraffin Sections (1:1000)

General description

Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).

This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.

Immunogen

Epitope: Within amino acids 131-136

human myelin basic protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.

Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197.Shin, R.W., et al. 1991. Lab. Invest.64, 693.

Packaging

100 µL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)